From the source of the Yarkon to Jerusalem.
Apx. 75 Km
Leave Rosh Ha-Ain following road # 444 to road # 443 towards Ben Shemen. From Ben Shemen, head towards the ancient cities of Lod and Ramle. From Ramle, head east on road # 424 through the Ayalon Valley to Latrun. Follow the old “Burma Road” (dirt road) along the rolling Judean hills to Mesilat Zion. Head to the village of Eshta'ol to road # 395 and start climbing to the hills of Jerusalem. Climb up to Ramat Raziel, towards the Sataf springs, descend to the Sorek valley and enter Jerusalem through Ein Kerem.
Off road option- From Mesilat Zion follow the dirt road through the “Bnei Brith” forest towards Mevaseret Zion and take the road to the Sataf, from there, follow the road to Ein Kerem.
Overnight in Jerusalem. An additional day or two is recommended in order to tour the city.