From the Jerusalem hills to the northern Negev.
Apx. 85 Km
Leave Jerusalem through Ein Kerem and follow road # 386 climbing to the southern part of the Jerusalem hills to Bar Giora. From Bar Giora, turn east on road # 3866 towards Beit Shemesh. From Beit Shemesh take road # 38 across the Judean plains to Lakhish. Enter the ruins of Lakhish and go south on the dirt road leading into the plains of the northern Negev, to the town of Lahav. From Lahav head east to road # 60 and take it south past Omer into Be'er Sheva, the capital of the Negev.
Over night in Be'er Sheva.